Question 1

Place the following steps for cloning DNA in the correct order:
 1 - use vector to deliver new rDNA to bacterial or other cells
2 - use restriction enzymes to cut a plasmid and add the desired gene
3 - isolate and cut out a desired gene using restriction enzymes
4 - use DNA ligase to seal the new gene
5 - allow bacterial cells to replicate and produce desired product

Accepted Answer

A)  1,2,3,4,5
B)  5,3,2,4,1
C)  2,5,3,1,4
D)  3,2,4,1,5E) All of the above
F) C) and D)

Question 2

What is the difference between ex vivo and in vivo gene therapy?

Accepted Answer

In ex vivo gene therapy,tissue such as b...

Question 3

Which of the following is mismatched?

Accepted Answer

A)  bioinformatics-the study of genomic and proteomic information using computer analysis
B)  polymerase chain reaction-process that separates DNA fragments according to size
C)  genomics-the study of the genomes of humans and other organisms
D)  proteomics-the study of species' proteinsE) B) and C)
F) A) and D)

Question 4

The function of a vector in genetic engineering is to

Accepted Answer

A)  cut DNA into many fragments.
B)  introduce rDNA into a host cell.
C)  link together newly joined fragments of DNA.
D)  make millions of copies of a specific segment of DNA.
E)  separate fragments of DNA by their length and electrical charges.F) B) and D)
G) C) and E)

Question 5

When using bacteria to clone a human gene and express its product,as in the case of insulin,the gene cannot contain introns because bacteria do not have the enzymes to process mRNA.How do scientists overcome this challenge?

Accepted Answer

Reverse transcriptase is used to "revers...

Question 6

Which of the following molecules forms lengths of DNA with "sticky ends"?

Accepted Answer

A)  DNA ligase
B)  DNA polymerase
C)  RNA polymerase
D)  reverse transcriptase
E)  restriction enzymeF) D) and E)
G) All of the above

Question 7

The term "ligase" adds the suffix "-ase" to the Latin root word for

Accepted Answer

A)  gene.
B)  work.
C)  cut.
D)  bind.
E)  copy-producing.F) C) and D)
G) A) and C)

Question 8

What is the function of gel electrophoresis in genetic engineering?

Accepted Answer

A)  cut DNA into many fragments
B)  carry DNA into a new cell
C)  link together newly joined fragments of DNA
D)  make millions of copies of a specific segment of DNA
E)  separate fragments of DNA by their length and electrical chargesF) A) and E)
G) C) and E)

Question 9

Which of the following statements about RNAi are true? Select all that apply.

Accepted Answer

A)  RNAi sequences are designed to be complementary to the rRNA that will function in the translation of a gene of interest.
B)  Once the complementary RNAi sequences enter the cell,they bind with the target RNA,producing double-stranded RNA molecules.
C)  These double-stranded RNA molecules are then broken down by a series of enzymes within the cell.
D)  RNAi is being researched as a potential treatment for a number of human diseases including cancer and hepatitis.E) B) and D)
F) A) and B)

Question 10

Biotechnology products produced by bacteria include all of the following EXCEPT

Accepted Answer

A)  antibodies to deliver radioisotopes to tumor cells.
B)  clotting factor VIII.
C)  human growth hormone.
D)  insulin.E) B) and C)
F) A) and D)

Question 11

A transgenic organism

Accepted Answer

A)  contains a foreign gene within its genome.
B)  is produced by cloning a mutant cell.
C)  acts as the vector for DNA being moved into another organism.
D)  is produced by the polymerase chain reaction.
E)  is any genetically modified organism resulting from laboratory research.F) A) and C)
G) A) and B)

Question 12

What consequences could arise if the field of bioinformatics ceased to exist as a field of study?

Accepted Answer

A)  Progress in determining the function of DNA sequences,making comparisons between our genome and other model organisms,and understanding how genes and proteins interact with cells would stop.
B)  Many cures for genetic disorders would not be discovered.
C)  The creation of new transgenic plants,animals,and bacteria that benefit society would not occur.
D)  All of these are consequences if the field of bioinformatics ceased to exist as a field of study.E) A) and B)
F) A) and C)

Exam 14: Biotechnology and Genomics

The function of a vector in genetic engineering is to

Correct Answer
verified

A transgenic organism

Correct Answer
verified

Biotechnology products produced by bacteria include all of the following EXCEPT

Correct Answer
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Which of the following statements about RNAi are true? Select all that apply.

Correct Answer
verified

Correct Answer
verified

What is the function of gel electrophoresis in genetic engineering?

Correct Answer
verified

Which of the following molecules forms lengths of DNA with "sticky ends"?

Correct Answer
verified

Which of the following is mismatched?

Correct Answer
verified

When using bacteria to clone a human gene and express its product,as in the case of insulin,the gene cannot contain introns because bacteria do not have the enzymes to process mRNA.How do scientists overcome this challenge?

Correct Answer
verified
Reverse transcriptase is used to "revers...
View Answer

What consequences could arise if the field of bioinformatics ceased to exist as a field of study?

Correct Answer
verified

What is the difference between ex vivo and in vivo gene therapy?

Correct Answer
verified
In ex vivo gene therapy,tissue such as b...
View Answer

The term "ligase" adds the suffix "-ase" to the Latin root word for

Correct Answer
verified

Exam 14: Biotechnology and Genomics

The function of a vector in genetic engineering is to

Correct AnswerverifiedShow Answer

A transgenic organism

Correct AnswerverifiedShow Answer

Biotechnology products produced by bacteria include all of the following EXCEPT

Correct AnswerverifiedShow Answer

Which of the following statements about RNAi are true? Select all that apply.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

What is the function of gel electrophoresis in genetic engineering?

Correct AnswerverifiedShow Answer

Which of the following molecules forms lengths of DNA with "sticky ends"?

Correct AnswerverifiedShow Answer

Which of the following is mismatched?

Correct AnswerverifiedShow Answer

When using bacteria to clone a human gene and express its product,as in the case of insulin,the gene cannot contain introns because bacteria do not have the enzymes to process mRNA.How do scientists overcome this challenge?

Correct AnswerverifiedReverse transcriptase is used to "revers...View AnswerShow Answer

What consequences could arise if the field of bioinformatics ceased to exist as a field of study?

Correct AnswerverifiedShow Answer

What is the difference between ex vivo and in vivo gene therapy?

Correct AnswerverifiedIn ex vivo gene therapy,tissue such as b...View AnswerShow Answer

The term "ligase" adds the suffix "-ase" to the Latin root word for

Correct AnswerverifiedShow Answer

Correct Answer
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Correct Answer
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Correct Answer
verified

Correct Answer
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Correct Answer
verified
Reverse transcriptase is used to "revers...
View Answer

Correct Answer
verified

Correct Answer
verified
In ex vivo gene therapy,tissue such as b...
View Answer

Correct Answer
verified