Question 1

Which of the following enzymes is needed to seal breaks in the sugar-phosphate backbone during replication?

Accepted Answer

A)   DNA polymerase
B)   RNA polymerase
C)   RNA ligase
D)   helicase
E)   DNA ligaseF) A) and B)
G) A) and C)

Question 2

Which of the following processes does not occur within the nucleus?

Accepted Answer

A)   DNA replication
B)   transcription
C)   intron removal
D)   translation
E)   primary mRNA splicingF) A) and B)
G) C) and E)

Question 3

Which of the following is not an application of polymerase chain reaction?

Accepted Answer

A)   production of many copies of an unknown piece of DNA
B)   making copies of a foreign gene for use in making recombinant DNA
C)   DNA fingerprinting of a crime suspect
D)   linking two DNA molecules together
E)   amplifying mitochondrial DNA sequencesF) A) and B)
G) A) and D)

Question 4

For a DNA strand that is 2 nucleotides long, how many different sequences are possible?

Accepted Answer

A)   2
B)   4
C)   8
D)   16
E)   64F) B) and D)
G) C) and D) D

Question 5

When DNA fingerprinting is performed, why might an individual possess two different numbers of repeats for the same chromosomal region?

Accepted Answer

A)   Intergenic DNA may vary from one cell to another in the same individual.
B)   The individual has different numbers of the same repeated sequence on each homologous chromosome.
C)   The sample is contaminated with the DNA of another individual.
D)   Each sister chromatid of a chromosome may contain different numbers of repeats.
E)   It is not possible for this to occur.F) A) and C)
G) A) and B)

Question 6

DNA replication makes a(n)  ____ copy of the DNA strand, while transcription makes a(n)  ____ copy of the DNA strand.

Accepted Answer

A)   mRNA; DNA
B)   DNA; mRNA
C)   DNA; tRNA
D)   mRNA; tRNA
E)   mRNA; rRNAF) B) and C)
G) A) and C) B

Question 7

Which of the following mutations would cause early termination of translation of the gene pictured?

Accepted Answer

A)   Change the 4th nucleotide of the top strand from C to T, and change the 4th nucleotide of the bottom strand from G to A
B)   Change the 5th nucleotide of the top strand from G to C, and change the 4th nucleotide of the bottom strand from C to G
C)   Change the 4th nucleotide of the top strand from C to A, and change the 4th nucleotide of the bottom strand from G to T
D)   Change the 6th nucleotide of the top strand from A to T, and change the 4th nucleotide of the bottom strand from T to A
E)   Change the last nucleotide of the top strand from A to G, and change the 4th nucleotide of the bottom strand from T to CF) All of the above
G) A) and B)

Question 8

Figure: 
-The sequence of the peptide that would result from transcribing and translating the gene pictured would be

Accepted Answer

A)   Tyr-Ala-Asn.
B)   Ile-Ser-Val.
C)   no peptide would be made, the first codon means "stop."
D)   Met-Arg-Leu.
E)   Tyr-Ser-Val.F) B) and E)
G) C) and D)

Question 9

_____ is the study of the structure and function of cellular proteins and their interactions; _______ may be employed to us computer algorithms to aid this process.

Accepted Answer

A)   Proteomics; bioinformatics
B)   Comparative genomics; bioinformatics
C)   Genomics; proteomics
D)   Proteomics; comparative genomics
E)   Comparative genomics; proteomicsF) All of the above
G) A) and E)

Question 10

Failure to remove introns from a primary mRNA would most likely cause

Accepted Answer

A)   irregularities in transcription.
B)   abnormal translation.
C)   DNA to mutate.
D)   DNA replication to fail.
E)   transcription to terminate early.F) A) and B)
G) None of the above

Question 11

If an organism's DNA contains 17% adenine nucleotides and 33% guanine nucleotides, what percentage of its DNA is composed of cytosine nucleotides?

Accepted Answer

A)   8.5%
B)   17%
C)   25%
D)   33%
E)   50%F) A) and E)
G) D) and E)

Question 12

Which base is found in DNA, but not in RNA?

Accepted Answer

A)   cytosine
B)   uracil
C)   thymine
D)   adenine
E)   guanineF) D) and E)
G) None of the above

Question 13

For a DNA strand that contains the sequence AGT in the 5' to 3' direction, what nucleotides are found on the other DNA strand in the 5' to 3' direction?

Accepted Answer

A)   ACT
B)   TGA
C)   AGT
D)   TCA
E)   GTAF) A) and B)
G) A) and C)

Question 14

Which of the following is most likely to cause no difference in phenotype between two individuals?

Accepted Answer

A)   variation of order of bases within a gene
B)   a transposon inserted into a gene
C)   possessing an extra copy of a gene
D)   a frameshift mutation within a gene
E)   variation of order of bases within an intergenic sequenceF) A) and E)
G) D) and E)

Question 15

For a DNA strand composed of the sequence AGT in the 5' to 3' direction, the free phosphate is on the ____ nucleotide and the free hydroxyl is on the ____ nucleotide.

Accepted Answer

A)   T; A
B)   A; G
C)   A; A
D)   G; T
E)   A; TF) A) and E)
G) B) and D)

Question 16

mRNA must be processed in the cytoplasm before being used in protein synthesis.

Accepted Answer

A) True 
 B)False

Question 17

Which of the following is an incorrect match?

Accepted Answer

A)   transcription - removal of introns
B)   translation - polypeptide formation
C)   transcription - tRNA formation
D)   translation - mRNA binding to rRNA
E)   replication - copying of DNAF) A) and E)
G) C) and E)

Question 18

Transgenic plants may have genes from animals inserted into their DNA.

Accepted Answer

A) True 
 B)False

Question 19

What chemical force must be overcome in order to separate the two DNA strands during replication?

Accepted Answer

A)   hydrophobic interactions
B)   intrastrand folding
C)   ionic bonds
D)   phosphodiester bonds
E)   hydrogen bondsF) A) and D)
G) C) and D) E

Question 20

The steps involved in a single PCR cycle, in the correct order, are

Accepted Answer

A)   cool to allow DNA strands to anneal, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
B)   heat to denature DNA strands and for primer to anneal, cool to allow DNA polymerase to copy DNA.
C)   heat to denature DNA strands, cool to allow primer to anneal and to allow DNA polymerase to copy DNA.
D)   cool to denature DNA strands, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
E)   cool to allow primer to anneal, heat to denature DNA strands, cool to allow DNA polymerase to copy DNA.F) B) and D)
G) B) and E)

Exam 11: DNA Biology and Technology

Which of the following enzymes is needed to seal breaks in the sugar-phosphate backbone during replication?

Correct AnswerverifiedShow Answer

Which of the following processes does not occur within the nucleus?

Correct AnswerverifiedShow Answer

Which of the following is not an application of polymerase chain reaction?

Correct AnswerverifiedShow Answer

For a DNA strand that is 2 nucleotides long, how many different sequences are possible?

Correct AnswerverifiedD

When DNA fingerprinting is performed, why might an individual possess two different numbers of repeats for the same chromosomal region?

Correct AnswerverifiedShow Answer

DNA replication makes a(n) ____ copy of the DNA strand, while transcription makes a(n) ____ copy of the DNA strand.

Correct AnswerverifiedB

Which of the following mutations would cause early termination of translation of the gene pictured?

Correct AnswerverifiedShow Answer

Figure: -The sequence of the peptide that would result from transcribing and translating the gene pictured would be

Correct AnswerverifiedShow Answer

_____ is the study of the structure and function of cellular proteins and their interactions; _______ may be employed to us computer algorithms to aid this process.

Correct AnswerverifiedShow Answer

Failure to remove introns from a primary mRNA would most likely cause

Correct AnswerverifiedShow Answer

If an organism's DNA contains 17% adenine nucleotides and 33% guanine nucleotides, what percentage of its DNA is composed of cytosine nucleotides?

Correct AnswerverifiedShow Answer

Which base is found in DNA, but not in RNA?

Correct AnswerverifiedShow Answer

For a DNA strand that contains the sequence AGT in the 5' to 3' direction, what nucleotides are found on the other DNA strand in the 5' to 3' direction?

Correct AnswerverifiedShow Answer

Which of the following is most likely to cause no difference in phenotype between two individuals?

Correct AnswerverifiedShow Answer

For a DNA strand composed of the sequence AGT in the 5' to 3' direction, the free phosphate is on the ____ nucleotide and the free hydroxyl is on the ____ nucleotide.

Correct AnswerverifiedShow Answer

mRNA must be processed in the cytoplasm before being used in protein synthesis.

Correct AnswerverifiedShow Answer

Which of the following is an incorrect match?

Correct AnswerverifiedShow Answer

Transgenic plants may have genes from animals inserted into their DNA.

Correct AnswerverifiedShow Answer

What chemical force must be overcome in order to separate the two DNA strands during replication?

Correct AnswerverifiedE

The steps involved in a single PCR cycle, in the correct order, are

Correct AnswerverifiedShow Answer

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D

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DNA replication makes a(n) copy of the DNA strand, while transcription makes a(n) copy of the DNA strand.

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B

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_ is the study of the structure and function of cellular proteins and their interactions; ___ may be employed to us computer algorithms to aid this process.

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For a DNA strand composed of the sequence AGT in the 5' to 3' direction, the free phosphate is on the nucleotide and the free hydroxyl is on the nucleotide.

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E

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